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microvascular endothelial cells  (ATCC)


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    Structured Review

    ATCC microvascular endothelial cells
    Microvascular Endothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microvascular+endothelial+cell+growth+kit+bbe/Microvascular+Endothelial+Cell+Growth+Kit-BBE/pm42875724-477-2-8
    Average 94 stars, based on 49 article reviews
    microvascular endothelial cells - by Bioz Stars, 2026-11
    94/100 stars

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    Cell Culture:

    Article Title: 3D-printed β-TCP scaffold as a bone-mimicking environment for an engineered model of osteosarcoma: In vitro properties and transcriptomic insights
    Article Snippet: .. Human dermal microvascular endothelial cell lines (TIME-GFP, CRL-4045, ATCC, USA) were cultured in Vascular Cell Basal Medium (ATCC, USA) with the addition of the Microvascular Endothelial Cell Growth Kit-BBE (ATCC, USA), 200 μg/mL G418 (G418 disulfate salt solution for cell culture; Sigma-Aldrich, USA) and 12.5 μg/mL Blasticidin (Blasticidin S HCl; Gibco, USA). .. One day before assembling the tri-culture on the scaffolds, the U2OS-MSC spheroids were stained with DiD (VybrantTM Multicolour Cell-Labelling Kit, Invitrogen, USA).

    Article Title: Evaluation of the metabolic activity, angiogenic impacts, and GSK-3β signaling of the synthetic cannabinoid MMB-2201 on human cerebral microvascular endothelial cells.
    Article Snippet: Human cerebral microvascular endothelial cells (HBEC5i) (CRL–3245) were obtained by ATCC (ATCC; Manassas, VA). .. These cells were cultured in DMEM/F12 media (Euroclone S.p.A, Pero, Italy) supplemented with Microvascular Endothelial Cell Growth Kit-BBE (PCS-110– 040; ATCC, Manassas, USA). ..

    Article Title: Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1
    Article Snippet: .. Primary human dermal microvascular endothelial cells (hMVEC-d) were purchased from Lonza (CC-2543), grown in EBM (CC-3156, Lonza) complete media (with supplements EGM TM -2 SingleQuots TM Supplements (CC-4176, Lonza)) at 37°C under 5% CO2, and used before passage five. hTERT-immortalized Dermal Microvascular Endothelial Cell, Neonatal (CRL4060, ATCC), was used. hTERT was cultured in Basal medium (PCS-100-030, ATCC) with the Microvascular Endothelial Cell Growth kit-BBE (PCS-110-040, ATCC) +0.5 ug/ml puromycin (P8833, Sigma-Aldrich) according to the manufacturer’s instruction. .. When indicated the following inhibitors were used: LY294002 (S1105, Selleckchem), U0123 (9903, Cell Signaling), cilengitide trifluoroacetate (S7077, Selleckchem), filipin III from Streptomyces filipinensis (480-49-9 Sigma-Aldrich), dynasore (304448-55-3, EMD millipore), desatinib (BMS-354825, S1021, Selleckchem), 2-Fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]-benzamide ((CK-666), SML0006, Sigma-Aldrich), imipramine hydrochloride (I0899, Sigma-Aldrich), SB203580, 5-(N-Ethyl-N-isopropyl) amiloride (1154-25-2, Sigma-Aldrich), ML141 (71203-35-5, EMD millipore), EHop-016 (S7319, Selleckchem) and NSC 23766 trihydrochloride (S8031, Selleckchem).

    Article Title: Evaluation of the metabolic activity, angiogenic impacts, and GSK-3β signaling of the synthetic cannabinoid MMB-2201 on human cerebral microvascular endothelial cells
    Article Snippet: Human cerebral microvascular endothelial cells (HBEC-5i) (CRL–3245) were obtained by ATCC (ATCC; Manassas, VA). .. These cells were cultured in DMEM/F12 media (Euroclone S.p.A, Pero, Italy) supplemented with Microvascular Endothelial Cell Growth Kit-BBE (PCS-110–040; ATCC, Manassas, USA). ..

    Article Title: IFITM1 inhibits Henipavirus membrane fusion by trapping ephrinB2 receptors in fusion-unfavorable membrane nanodomains
    Article Snippet: HeLa cells, PK13 cells, and Human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich D6429) with 10% fetal bovine serum (FBS, Thermo Fisher 12483-020). .. Human microvascular endothelial cells (HuMEC) (ATCC CRL-4060) were cultured in Vascular cell basal medium (ATCC, PCS-100-030) with Microvascular endothelial cell growth kit – BBE (ATCC, PCS-110-040) and 0.5 ug/ml puromycin (10 mg/ml stock, Gibco A1138-03). .. Cells were washed with phosphate-buffered saline (PBS, Thermo Fisher Scientific, 10010049) and detached by 0.25% trypsin-EDTA (Thermo Fisher Scientific, 25200072) before seeding and sub-culturing.

    Article Title: Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1.
    Article Snippet: .. Primary human dermal microvascular endothelial cells (hMVEC-d) were purchased from Lonza (CC-2543), grown in Basal Medium (EBM-2) (CC-3156, Lonza) complete media (with supplements EGMTM-2 SingleQuotsTM Supplements [CC-4176, Lonza]) at 37°C under 5% CO2 and used before passage five. hTERT-immortalized dermal microvascular endothelial cell, neonatal (CRL4060, ATCC), was used. hTERT was cultured in Vascular Cell Basal Medium (VCBM) (PCS-100-030, ATCC) with the microvascular endothelial cell growth kit-BBE (PCS-110-040, ATCC) +0.5 μg/mL puromycin (P8833, Sigma-Aldrich) according to the manufacturer’s instruction. .. When indicated, the following inhibitors were used: LY294002 (S1105, Selleckchem), U0123 (9903, Cell Signaling), cilengitide trifluoroacetate (S7077, Selleckchem), filipin III from Streptomyces filipinensis (48049-9 Sigma-Aldrich), dynasore (304448-55-3, EMD Millipore), desatinib (BMS-354825, S1021, Selleckchem), 2-Fluoro-N-[2-(2-methyl-1Hindol-3-yl)ethyl]-benzamide ([CK-666], SML0006, Sigma-Aldrich), imipramine hydrochlor ide (I0899, Sigma-Aldrich), SB203580, 5-(N-ethyl-N-isopropyl) amiloride (1154-25-2, Sigma-Aldrich), ML141 (7120335-5, EMD Millipore), EHop-016 (S7319, Selleckchem) and NSC 23766 trihydrochloride (S8031, Selleckchem).

    Derivative Assay:

    Article Title: The tubule-sheet continuum model links the pattern of nuclear envelope assembly to nuclear envelope integrity
    Article Snippet: Normal mammalian cell lines : NRK-52E and NIH-3T3 derived cell lines were grown in DMEM+GlutaMAX supplemented with 10% FBS. hTERT-immortalized human primary cell lines : RPE-1 derived cell lines were grown in in DMEM: F12 without phenol red (Gibco 21041-025) supplemented with 10% FBS. .. Dermal Microvascular Endothelial (DMVE) Cell, Neonatal derived cell lines were grown in Vascular Cell Basal medium supplemented with the Microvascular Endothelial Cell Growth Kit-BBE (ATCC PCS-110-040). .. HPNE cells were grown in a 3:1 mixture of DMEM+GlutaMAX: Medium M3 Base supplemented with 10% FBS and 10 ng/ml human recombinant EGF (StemCell Technologies Inc. 78006).

    other:

    Article Title: Transendothelial migration of the Lyme disease spirochete involves spirochete internalization as an intermediate step through a transcellular pathway that involves Cdc42 and Rac1
    Article Snippet: Primary human dermal microvascular endothelial cells (hMVEC-d) were purchased from Lonza (CC-2543), grown in Basal Medium (EBM-2) (CC-3156, Lonza) complete media (with supplements EGMTM-2 SingleQuotsTM Supplements [CC-4176, Lonza]) at 37°C under 5% CO 2 and used before passage five.



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